Assessment of a fruit extract (Sechium edule) on the labeling of blood elements with technetium-99m

Dublin Core

Title

Assessment of a fruit extract (Sechium edule) on the labeling of blood elements with technetium-99m

Subject

Full Length Research Paper

Description

Natural products have been widely used by human beings. However, sometimes the biological effects of these products are not fully known. Chayotte (Sechium edule) is a vegetable used in the folk medicine. Red blood cells (RBC) labeled with technetium-99m (99mTc) have several clinical applications. The aim of this work was to evaluate the influence of an extract of chayotte on the labeling of blood elements with 99mTc using stannous chloride (SnCl2) in the concentrations like to 1.2, 0.006, 0.0005 and 0.0006 mg/ml. The extract of chayote was incubated in various concentrations for 1hour with blood which was withdrawn from Wistar rats. After that SnCl2 was added and the incubation continued for more 1 h. Elapsed this time 99mTc as sodium pertechnetate (NaTcO4) was toted. The blood was centrifuged and plasma (P) and RBC were isolated, also precipitated with trichloroacetic acid (TCA, 5%) and soluble (S) and insoluble (I) fractions (F) of plasma and cells (C) were determined. The radioactivity (ATI%) was rated in RBC, IF-P and IF-C. The results have showed that extract was able to reduce the radiolabeling using SnCl2 (0.006, 0.0005 and 0.0006mg/ml). We can speculate that this effect may be on account of the products with oxidant proprieties.



Key words: chayote, red blood cells, plasma proteins, technetium-99m, radiopharmacy.

Creator

Glaacute;ucio Direacute; Feliciano, Maria Luiacute;sa Gomes, Elaine Alves Correia Lima, Roberto Levi Jales, Mauro Castro Faria and Maacute;rio Bernardo Filho

Publisher

Academic Journals

Date

2004

Rights

Copyright © 2004 Glaacute;ucio Direacute; Feliciano, Maria Luiacute;sa Gomes, Elaine Alves Correia Lima, Roberto Levi Jales, Mauro Castro Faria and Maacute;rio Bernardo Filho

Format

text/html

Language

en

Type

TEXT

Identifier

https://academicjournals.org/journal/AJB/article-abstract/89796CB32808
http://dx.doi.org/10.5897/AJB2004.000-2095

Citation

Glaacute;ucio Direacute; Feliciano, Maria Luiacute;sa Gomes, Elaine Alves Correia Lima, Roberto Levi Jales, Mauro Castro Faria and Maacute;rio Bernardo Filho, “Assessment of a fruit extract (Sechium edule) on the labeling of blood elements with technetium-99m,” Open Access Journal Archives, accessed August 2, 2021, http://oajour.info/items/show/711.